Ke Zhou12 Jian Yang2 Zao-Xia Wang2 Ji-Rui Wang2 *
1.College of AgronomySichuan Agricultural UniversityChengduChina
2.Triticeae Research InstituteSichuan Agricultural UniversityChengduChina
*. Email author
Ke Zhou and Jian Yang are Co-first authors.
Genes & Genomics, February 2017, Volume 39, Issue 2, pp 161–171
Abstract
Pre-harvest sprouting
(PHS) is the germination of grains in the spike under wet conditions
before harvest, which causes a severe reduction in crop yield and flour
quality. The phytohormone abscisic acid plays a key role in regulating
seed dormancy, which inhibits pre-harvest spouting or vivipary for many
important crops. TaABI5, belonging to a family of basic leucine zipper
transcription factors could regulate a subset of late embryogenesis
abundant genes during seed development, was isolated and characterized.
Phylogenetic analysis revealed that TaABI5 homologous gene belonged to
the ABI5 subgroup of the ABF/AREB/ABI5 subfamily. TaABI5 consist of four
conserved domains and one bZip domain. The expression pattern of TaABI5
indicated that it was seed-specific and accumulated at late stages of
seed development. Accumulation of TaABI5 in synthetic hexaploid wheat
SHW-L1 (PHS resistance) was 8.93-fold higher than that in wheat cultivar
Chuanmai 32 (PHS susceptible) at 25 days post anthesis. Four expression
quantitative trait loci (eQTL) of TaABI5 on chromosome 2DS, 4DS, 6DS,
and 7DL were characterized from SHW-L1/Chuanmai 32 derived recombinant
inbred line population.
Keywords
Pre-harvest sprouting ABI5 Phylogenetic tree Expression